Radioimmunoassay for the Measurement of Pregnancy-Associated Plasma Protein-A (PAPP-A) in Humans

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Radioimmunoassay for pregnancy-associated plasma protein A.

A specific and highly sensitive radioimmunoassay for determination of pregnancy-associated plasma protein A in human serum is described. The minimum detection limit for this protein was 2.9 micrograms/L. The within- and between-assay coefficients of variation were 4.0 and 4.5%, respectively. The circulating protein was detected within 32 days of conception in eight normal pregnancies and within...

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Pregnancy-associated plasma protein-E (PAPP-E).

A full-length cDNA encoding a novel human protein was cloned from placenta cDNA. The corresponding 1542 amino acid protein sequence was termed 'pregnancy-associated plasma protein-E' (PAPP-E) as it shows a 62% homology to the human pregnancy-associated plasma protein-A (PAPP-A) that is a diagnostic marker for trisomies, especially Down syndrome. The conserved domain structure contains five moti...

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Pregnancy-associated plasma protein-E (PAPP-E)1

A full-length cDNA encoding a novel human protein was cloned from placenta cDNA. The corresponding 1542 amino acid protein sequence was termed `pregnancy-associated plasma protein-E' (PAPP-E) as it shows a 62% homology to the human pregnancy-associated plasma protein-A (PAPP-A) that is a diagnostic marker for trisomies, especially Down syndrome. The conserved domain structure contains five moti...

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Immunoassays developed for pregnancy-associated plasma protein-A (PAPP-A) in pregnancy may not recognize PAPP-A in acute coronary syndromes.

BACKGROUND Pregnancy-associated plasma protein-A (PAPP-A) concentrations are increased in the circulation of patients with acute coronary syndromes (ACS) and are associated with future adverse cardiac events. PAPP-A in ACS differs from PAPP-A in pregnancy in that PAPP-A in ACS is not complexed with the proform of eosinophil major basic protein (proMBP). We investigated the effect of antibody se...

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Comment on immunoassays developed for pregnancy-associated plasma protein-A (PAPP-A) in pregnancy may not recognize PAPP-A in acute coronary syndromes.

1. Wald NJ, Cuckle H, Nanchahal K. Amniotic fluid acetylcholinesterase measurement in the prenatal diagnosis of open neural tube defects. Second Report of the Collaborative Acethylcholinesterase Study. Prenat Diagn 1989;9:813–29. 2. Canick JA, Kellner LH, Bombard AT. Prenatal screening for open neural tube defects. Lab Med 2003;23:385–94. 3. Hoffman A, Nimtz M, Getzlaff R, Conradt HS. “Brain ty...

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ژورنال

عنوان ژورنال: Biology of Reproduction

سال: 1981

ISSN: 0006-3363,1529-7268

DOI: 10.1095/biolreprod24.5.1076